How it Works
How it Works
- A liquid biopsy (blood sample) is taken from the patient – typically 10ml in an EDTA vacutainer tube
- The sample is attached to the Parsortix instrument. No pre-processing is required.
- A Parsortix filtration cassette is inserted into the instrument and the system is primed for use.
- The Parsortix instrument automatically passes the blood through the filtration cassette.
- CTCs are caught in the Parsortix cassette due to their larger size and lower compressibility than other blood components.
- Once caught in the cassette….
- CTCs can be automatically stained inside the cassette with selected antibodies. This enables their identification and characterisation. CTCs can be enumerated by placing the filtration cassette under a fluorescent microscope.
- Alternatively, cells can be eluted from the cassette and recovered in 200ul of buffer solution. Cells can now be analysed in a range of ways including qPCR, FISH, IHC and can even be cultured.
- Information obtained from this liquid biopsy has the potential to be used to inform clinicians about the patient’s condition and most appropriate treatment. Note: The Parsortix system is for research use only (RUO) and is not for use in diagnostic procedures.