Portrait®+ CTC Staining Kit

Reveal more with the illuminating power of CTCs

Shine a new light on cancer cells with the combined power of the Portrait®+ CTC staining kit and Parsortix®technology

Portrait+ CTC staining kit

  • Provides advanced immunofluorescent staining of CTCs enriched from blood by Parsortix technology
  • Enables the identification and enumeration of epithelial and mesenchymal cells including those undergoing epithelial-to-mesenchymal transition (EMT)
  • EMT is a key transition step in cancer cells, and is associated with tumor progression, the development of drug resistance, and metastasis1-3

Discover a new level of clarity

The Portrait+ CTC staining kit uses direct staining and an optimized, vivid dye combination, to ensure high signal intensity while maintaining high analytical specificity and sensitivity.

  • Visualize CTCs from multiple cancer types, including ovarian, breast, lung and prostate
  • Provides insights to help you make more informed decisions about further downstream analysis

Identify circulating tumor cells (CTCs) with the Portrait+ CTC staining kit and Parsortix technology

Easy to use, reliable results

Antibodies are supplied pre-mixed and lyophilized for ease-of-use and long-term storage

  • Have confidence with consistent, reliable results from fully validated, standardized protocols
  • Provides an alternative to costly and time-consuming in-cassette staining

Efficiency without compromise

The Portrait+ CTC staining kit includes the CellKeep™ slide, a unique CTC harvesting technology to maximize the retention of CTCs enriched from blood samples.

  • Confines the harvested CTCs to a small area, reducing the volume of antibodies required for staining and decreasing imaging time
  • Helps drive process efficiency and minimize cost

Example images from patient blood samples

 

White = white blood cell marker (Cy5), blue = nucleus (DAPI), red = mesenchymal marker (Cy3), green = epithelial marker (FITC).

Examples of Portrait+ CTC staining kit samples

Examples of Portrait+ CTC staining kit samples

 

Healthy volunteer blood samples spiked with SKBR3 and Hs 578T cancer cell lines.

White = white blood cell marker (Cy5), blue = nucleus (DAPI), red = mesenchymal marker (Cy3), green = epithelial marker (FITC).

Portrait+ CTC Staining kit analytical sensitivity and analytical specificity*

Key performance metrics using Hs 578T (mesenchymal) and SKBR3 (epithelial) cell lines spiked and recovered from peripheral blood using Parsortix technology. Mean analytical sensitivity and analytical specificity.

Epithelial

97%

Analytical Sensitivity

Epithelial

95%

Analytical Specificity

Mesenchymal

83%

Analytical Sensitivity

Mesenchymal

92%

Analytical Specificity

For Research Use Only. Not For Use in Diagnostic Procedures.

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    * Analytical sensitivity = proportion of spiked cells known to express the marker(s) of interest which were marker positive in the assay. Analytical specificity = proportion of spiked cells known to NOT express the marker(s) of interest which were marker negative in the assay.

     

    References

     

    1. Mittal, V. Epithelial Mesenchymal Transition in Tumor Metastasis. Annu. Rev. Pathol. 13, 395–412 (2018). 2. Silvestri, M. et al. Copy number alterations analysis of primary tumor tissue and circulating tumor cells from patients with early-stage triple negative breast cancer. Sci. Rep. 12, 1470 (2022). 3. Payne, K. et al. Characterizing the epithelial–mesenchymal transition status of circulating tumor cells in head and neck squamous cell carcinoma. Head Neck 44, 2545–2554 (2022).